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Fig. 4 | BMC Cancer

Fig. 4

From: A role for ceruloplasmin in the control of human glioblastoma cell responses to radiation

Fig. 4

Modulation of cellular viability and apoptosis in CP-modulated cells post-IR. Early and late apoptosis was evaluated by measure of annexin V positive cells and Ann V and IP positive cells, respectively, by flow cytometry in stable transfected U251-MG (A) and U87-MG GB (B) cell lines 24 h after a single exposure to 4 Gy- or 16 Gy-irradiation at 3% oxygen. Apoptosis is expressed as relative MFI of annexin V and PI ± SEM (n = 3). In same conditions but here 48 h after IR, MTS viability assay was performed in U251-MG (C) and U87-MG cells (D). MTS viability is expressed as DO ± SEM (n = 7). One-way ANOVA with multiple comparisons (each group was compared to their own normalized control) was performed between Control vs. irradiated cells (*, p-value ≤ 0.05; **, p-value ≤ 0.01; ***, p-value ≤ 0.001; ****, p-value ≤ 0.0001)

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