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Fig. 2 | BMC Cancer

Fig. 2

From: A role for ceruloplasmin in the control of human glioblastoma cell responses to radiation

Fig. 2

Change in expression of iron metabolism-related genes and ceruloplasmin protein in response to IR in U251-MG and U87-MG cells. mRNA level fold change of transferrin receptor 1 (TFR1), divalent metal transporter 1 (DMT1), heavy and light ferritin chain (respectively FTH1 and FTL), Mitoferrin 1 and 2 (respectively MFRN1 and MFRN2), frataxin (FXN), ferroportin (FPN1) and CP in U251-MG (A and C) and U87-MG (B and D) GB cells cultivated at 21% or 3% oxygen at 3- (A and B) or 8- days (C and D) post-IR with 4 Gy. Fold change are expressed as mean ± SEM (n = 3) and relative to non-irradiated control condition, given the arbitrary value of 1. Multiple T-Test was performed (*, p-value ≤ 0.05 for 21%; # p-value ≤ 0.05 for 3%). Levels of CP protein and heat shock cognate protein 70 (HSC70) protein used as loading control protein in U251-MG (E) and U87-MG (F) GB cells cultivated at 21% or 3% oxygen in non-irradiated condition (0) or 3 days after an irradiation of 4 Gy. Western blot data represent one of three independent experiments with comparable results

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