Skip to main content
Fig. 5 | BMC Cancer

Fig. 5

From: Targeting autophagy increases the efficacy of proteasome inhibitor treatment in multiple myeloma by induction of apoptosis and activation of JNK

Fig. 5

Autophagy inhibitors in combination with ixazomib induce apoptosis and arrest cell cycle at G1 phase in multiple myeloma cells. KMS11 cells were treated with ixazomib and autophagy inhibitors as described above. A Apoptosis was measured by analysis of apoptosis fraction using annexin-V/PI staining. (BAF, 5 nM) (n = 3) or chloroquine (CQ, 40 μM), (n = 2). P values were calculated by one-way analysis of variance (ANOVA). A P value of less than 0.05 was considered statistically significant (*p < 0.05, **p < 0.01, ***p < 0.001). B Using western blot protein levels of caspase3, cleaved-caspase3, PARP, cleaved PARP, p21CIP1 and p27.KIP1 were measured. β-actin was usedas loading control, (n = 3)

Back to article page