Fig. 3

Development of 3D spheroids of CD20⁺ B-cell lymphomas and expression pattern of CD59. a Inverted microscopy images of 3D cultures of Raji, GRANTA-519, Daudi, and BJAB cells after 1-4 days of culture (magnification 5x) (left), and corresponding growth curves showing mean diameter of 5 spheroids per time point (right). b Confocal image z-stack projection of a representative GRANTA-519 spheroid stained with anti-CD59 PE antibody (red) (scale bar = 300 μm, magnification 10x). Profile of the CD59 intensity (yellow line) was examined. The X-axis represents distance along the spheroid and the Y-axis the pixel intensity