Fig. 2
RTX-mediated CDC in 2D cultures of CD20+ B-cell lymphomas. Cell viability was analyzed by trypan blue exclusion in four B-cell lymphoma cell lines cultured in monolayers and supplemented with human plasma, or where indicated, with heat-inactivated (HI) plasma. a Percentage cell viability in Raji, Daudi, BJAB, and GRANTA-519 cells following treatment with RTX isotypes or isotype controls. b Percentage cell viability with or without CD59 blocking, using 50 or 25 μg/mL anti-human CD59 (p282) in BJAB and GRANTA-519 cells respectively, prior treatment with RTX isotypes. Data are presented as the mean percentage cell viability ± SEM of 3-6 independent experiments. Statistical significance was determined by one-way ANOVA with Tukey post hoc test for multiple comparisons. Asterisk represents significant difference between RTX and isotype control, or between RTX treatments (with and without CD59 blocking)