Fig. 1

CD20 and CD59 expression in four B-cell lymphoma cell lines. Raji, Daudi, BJAB, and GRANTA-519 cells were cultured in 2D monolayers and stained with anti-CD20 or anti-CD59 antibody, and analyzed by flow cytometry. Representative histograms of cells stained with phycoerythrin (PE)-labeled anti-CD20 (a) and anti-CD59 (b) antibody at passage number 11/12. Grey histogram represents isotype control. Graph represents the median fluorescence intensity (MFI) of CD20 (a) and CD59 (b) on passage number 2-15 with corresponding isotype control subtracted. c Mean of MFI of passage number 2-15 with corresponding isotype control subtracted ± SEM of 8 independent experiments, and ratio of mean MFI of CD20 and CD59