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Fig. 1 | BMC Cancer

Fig. 1

From: Circulating tumour cell gene expression and chemosensitivity analyses: predictive accuracy for response to multidisciplinary treatment of patients with unresectable refractory recurrent rectal cancer or unresectable refractory colorectal cancer liver metastases

Fig. 1

Representative: A) qRT-PCR CTC validation and B) accompanying histogram, demonstrating β-actin, CK18 and CK19 but not CD45, CD31 or N-Cadherin mRNA expression in a 6-day CTC culture, plus C) IF validation demonstrating positive IF immunoreactivity for EpCAM, Pan-CK but not CD45 in a 6 day CTC culture (left panels), and positive IF immunoreactivity for CD45 but not EpCAM or Pan-CK in PBMCs from the same patient (right panels) (bar = 50 μm)

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