Table 2 Antibodies and concentrations used in Western Blot and IHC in this study
From: CKAP2L, a crucial target of miR-326, promotes prostate cancer progression
Antibodies | Source, Identifier | Western blot | IHC |
|---|---|---|---|
CKAP2L | Thermo Fisher Scientific, PA5-58,778 | 1:1000 | 1:200 |
MAD2L1 | Abcam, ab10691 | 1:500 | / |
PLK1 | Abcam, ab189139 | 1:1000 | / |
AURKB | Abcam, ab45145 | 1:5000 | / |
BIRC5 | Abcam, ab76424 | 1:5000 | / |
CCND1 | Abcam, ab16663 | 1:200 | / |
SMC4 | Cell Signaling Technology, 5547S | 1:1000 | / |
KIF2C | Abcam, ab187652 | 1:1000 | / |
GAPDH | Abcam, ab181602 | 1:10,000 | / |
Cleaved Caspase-3 | Abcam, ab2302 | / | 1:10 |
Ki-67 | Abcam, ab16667 | / | 1:200 |