Investigation of BRCAness associated miRNA-gene axes in breast cancer: cell-free miR-182-5p as a potential expression signature of BRCAness

Table 1 The primer sequences which used in RT-PCR and quantitative real-time PCR

Primer name	Primer Sequence (5′ → 3′)
miR-182-5p	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGTGTGAG
	F: GTTTGGCAATGGTAGAACTCACAC
	R: GTGCAGGGTCCGAGGT
miR-146a-5p	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACCCA
	F: CGAACTGAGAACTGAATTCCA
	R: GTGCAGGGTCCGAGGT
miR-498	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAAAAA
	F: TATATTTCAAGCCAGGGGGCG
	R: GTGCAGGGTCCGAGGT
BRCA1	F: CCCTCAAGGAACCAGGGATG
BRCA1	R: GCTGCACGCTTCTCAGTGGT
BRCA2	F: AGACTGTACTTCAGGGCCGTACA
BRCA2	R: GGCTGAGACAGGTGTGGAAACA
BRIP1	F: CAACTATCCAAGCACACCACC
BRIP1	R: AACTTTGCAGCCAGAGTGGTT
BARB1	F: ACAACTGGACAGCATGATTCAAC
BARB1	R: ACTTCGAGGGCTAAACCACA
RAD51	F: CCAGACCCAGCTCCTTTATC
RAD51	R: CACTGCTACACCAAACTCATC
CHEK2	F: TCAGCAAGAGAGGCAGACCC
CHEK2	R: ACAGCTCTCCCCCTTCCATC
PUM1	F: AGTGGGGGACTAGGCGTTAG
PUM1	R: GTTTTCATCACTGTCTGCATCC
RNU44	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGTCAGTT
	F: GAATGCTGACTGAACATGAAGGTC
	R: GTGCAGGGTCCGAGGT

ISSN: 1471-2407