Comprehensive bioinformatic analysis reveals a cancer-associated fibroblast gene signature as a poor prognostic factor and potential therapeutic target in gastric cancer

Table 4 Parameters of the stepwise multivariate Cox proportional regression model for cancer-associated fibroblasts and signature genes. TCGA data for stomach adenocarcinoma, adrenocortical carcinoma, kidney renal papillary cell carcinoma, and mesothelioma were analyzed in the TIMER2.0 immune association - outcome module using the TIDE algorithm for the allocation of samples to the high vs. low CAF infiltration groups. Likelihood ratio and Score log-rank tests were performed (CAF: cancer-associated fibroblast, CI: confidence interval, HR: hazard ratio)

Variate/s	HR of CAF infiltration	95% CI	z-score	p-value
	Stomach Adenocarcinoma
CAF, *COL1A1, COL5A1*	11.654	2.247–60.438	2.924	0.003
CAF, *COL1A1, COL5A1, ITGA4*	12.247	2.335–64.240	2.963	0.003
CAF, *COL1A1, COL5A1, ITGA4, EMILIN1*	28.315	4.143–193.524	3.409	0.001
CAF, *COL1A1, COL5A1, ITGA4, EMILIN1, TSPAN9*	36.813	5.007–270.645	3.543	0.000
	Adrenocortical Carcinoma
CAF, *COL1A1, COL5A1*	0.011	0.000–10.094	−1.297	0.195
CAF, *COL1A1, COL5A1, ITGA4*	0.011	0.000–11.744	−1.270	0.204
CAF, *COL1A1, COL5A1, ITGA4, EMILIN1*	0.007	0.000–9.801	−1.341	0.180
CAF, *COL1A1, COL5A1, ITGA4, EMILIN1, TSPAN9*	0.014	0.000–23.333	−1.126	0.260
	Kidney Renal Papillary Cell Carcinoma
CAF, *COL1A1, COL5A1*	0.002	0.000–0.223	−2.566	0.010
CAF, *COL1A1, COL5A1, ITGA4*	0.002	0.000–0.241	−2.535	0.011
CAF, *COL1A1, COL5A1, ITGA4, EMILIN1*	0.002	0.000–0.287	−2.427	0.015
CAF, *COL1A1, COL5A1, ITGA4, EMILIN1, TSPAN9*	0.002	0.000–0.515	−2.197	0.028
	Mesothelioma
CAF, *COL1A1, COL5A1*	0.000	0.000–0.128	−2.513	0.012
CAF, *COL1A1, COL5A1, ITGA4*	0.000	0.000–0.166	−2.443	0.015
CAF, *COL1A1, COL5A1, ITGA4, EMILIN1*	0.000	0.000–0.737	−2.036	0.042
CAF, *COL1A1, COL5A1, ITGA4, EMILIN1, TSPAN9*	0.001	0.000–0.133	−2.834	0.005

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