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Fig. 5 | BMC Cancer

Fig. 5

From: MIF/CXCR4 signaling axis contributes to survival, invasion, and drug resistance of metastatic neuroblastoma cells in the bone marrow microenvironment

Fig. 5

BM-like culture conditions increase CXCR4 surface expression and activate AKT and ERK pathways. A) Flow cytometry histograms of CXCR4 expression in LAN-1 cells cultured under BM-like conditions for 72 h (left). CXCR4 expression is represented as a fold-change to CM-CNT Nx (n = 3; right). B) Flow cytometry histograms of CXCR4 expression in SH-SY5Y cells cultured under BM-like conditions for 72 h (left). CXCR4 expression is represented as a fold-change to CM-CNT Nx (n = 3; right). Histograms were generated with NovoFlow Software (Acea Bioscience). Normoxia results are in black and hypoxia in blue. (*) P < 0.01 (Two-way ANOVA, Dunnett’s multiple comparison test). Immunoblots of LAN-1 C) and SH-SY5Y D) cultured under BM-like conditions for 48 h. Band intensity p-AKT (S473), p-ERK (T202/Y204 T185/Y187) ratios are calculated by measuring the relative protein levels (phosphorylated/total), and γ-Tubulin (as a loading control) by using ImageJ Software (n = 2)

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BMC Cancer

ISSN: 1471-2407

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