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Fig. 3 | BMC Cancer

Fig. 3

From: MIF/CXCR4 signaling axis contributes to survival, invasion, and drug resistance of metastatic neuroblastoma cells in the bone marrow microenvironment

Fig. 3

BM-like culture conditions promote NB proliferation, invasion, and drug resistance. A) LAN-1, SH-SY5Y, IMR5, and HSJD-NB01 cells were cultured in CMs under normoxic, and hypoxic conditions and MTS assay was performed over 4 days. Data are represented as a fold change viability to time 0 h (n = 6). B) Wound healing assay of LAN-1 cells after 24 h (left) and its quantification (right). Differences were calculated comparing each experimental condition to control (CM-CNT in normoxia) (n = 3). Normoxia conditions (black) hypoxia conditions (blue). Two-way Anova, Dunnett’s multiple comparison test. C) Transwell invasion assay of LAN-1 cells after 96 h (left) and its quantification (right). Differences were calculated comparing each experimental condition to control (CM-CNT in normoxia) (n = 3). Two-way Anova, Dunnett’s multiple comparison test. D) Activity of chemotherapy (doxorubicin, etoposide, and SN-38) over LAN-1 cells cultured with CM-BM/NB at 72 h (n = 3). Normoxia conditions (black) hypoxia conditions (blue). (*) P < 0.05

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