Fig. 4

Reducing the expression of EEF1D gene significantly aggravated the pathological changes of SKOV3/DDP xenografted tumor and increased the apoptosis of SKOV3/DDP ovarian cancer cell in xenografted tumor induced by DDP. A HE-stained tissue of xenografted tumor; a: control (× 400), b: DDP (× 400), c: DDP + scrambled shRNA (× 400), d: DDP + EEF1D shRNA (× 200). B The tumor tissues stained with TUNEL kit (fluorescein-dUTP) and observed under inverted fluorescence microscope (× 400); a: control, b: DDP, c: DDP + scrambled shRNA, d: DDP + EEF1D shRNA. C Apoptosis was quantified by Image J software. Data are presented as mean ± SD of six mice in each group. ^**P < 0.01 vs. control; ^##P < 0.01 vs. DDP group. HE: hematoxylin and eosin