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Fig. 9 | BMC Cancer

Fig. 9

From: Cystatin SN promotes epithelial-mesenchymal transition and serves as a prognostic biomarker in lung adenocarcinoma

Fig. 9

Knockdown of CST1 inhibits EMT in A549 and H1299 cells. a, d The mRNA levels of CST1 in CST1-silenced A549 and H1299 cells were detected by q-PCR. b, e The protein levels of CST1 in CST1-silenced A549 and H1299 cells were detected by western blotting. c, f The expression of EMT markers was detected by western blotting. g, h Wound-healing migration assays of CST1-silenced A549 and H1299 cells as well as control cells, which were treated as described above. i-j CST1-silenced A549 and H1299 cells were allowed to migrate through an 8-μM-pore size Transwell insert. Knockdown of CST1 inhibited the migration and invasion abilities of A549 and H1299 cells. Migrated and invaded cells were stained and counted in at least three microscopic fields. *p < 0.05, **p < 0.01 ***p < 0.001. (The blots were cutted prior to hybridisation with antibodies)

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