Fig. 8

Overexpression of CST1 promotes EMT in A549 and H1299 cells. a, d The mRNA levels of CST1 in A549 and H1299 cells overexpressing CST1 were detected by q-PCR. b, e The protein levels of CST1 in A549 and H1299 cells overexpressing CST1 were detected by western blotting. c, f The expression of EMT markers was detected by western blotting. g, h Wound-healing migration assays of A549 and H1299 cells overexpressing CST1 as well as control cells, which were treated as described above. i-j A549 and H1299 cells overexpressing CST1 were allowed to migrate through an 8-μM-pore size transwell insert. Overexpression of CST1 inhibits the migration and invasion abilities of A549 and H1299 cells. Migrated and invaded cells were stained and counted in at least three microscopic fields. *p < 0.05, **p < 0.01 ***p < 0.001. (The blots were cutted prior to hybridisation with antibodies)