Fig. 4From: Integrative analysis of cell adhesion molecules in glioblastoma identified prostaglandin F2 receptor inhibitor (PTGFRN) as an essential genePTGFRN is upregulated in GSCs and required for its growth. A Heatmaps representing the differentially expressed CAMs in GSCs as compared to NSCs (left) and in GSCs as compared to DGCs (right). GSE46016 (GSC vs NSC, gene microarray) and GSE54791 (GSC vs DGC, RNA-Seq) datasets were used for the analysis. Red and green color indicates the upregulated and downregulated CAMs, respectively. B Scatter plots represent the transcript levels of PTGFRN in different datasets GSE119834, GSE31262, and GSE54791. C Immunoblot shows the protein levels of PTGFRN in GSCs and corresponding DGCs in MGG8, MGG6, MGG4, and 1035 and β-Actin was used as a loading control (required portion of the blot is shown after cropping from the whole blot for both the proteins). GSCs cultured as neurospheres indicated as Sph (Spheroid culture) and DGCs cultured as monolayer and indicated as Diff (Differentiated cells). D The bar graph shows the transcript levels of PTGFRN in shNT and shPTGFRN in MGG6 and MGG8. E Representative images of neurospheres and their quantification in MGG6 and MGG8 after silencing PTGFRN. F Line graphs show the limiting dilution analysis in MGG6 and MGG8 after silencing PTGFRN. G Bar diagram shows the transcript levels of Olig2, Pou3f2, Sall2, and Sox2 in shNT and shPTGFRN (represented as shPT) in MGG6 and MGG8. The significance was performed using the Wilcoxon-Mann-Whitney test or Student’s t-test and the symbols are indicated as follows: (ns) not significant; (*) p ≤ 0.05; (**) p ≤ 0.01 and (***) p ≤ 0.001Back to article page