Fig. 3

Regulation of PTGFRN by promoter methylation and miR-137. A Scatter plots depicting the beta values for CpGs cg22448232 in control and GBM samples in TCGA 450 K, GSE60274, and GSE79122 datasets. B The correlation graph shows the correlation between CpG methylation of cg22448232 and the expression of PTGFRN in TCGA GBM samples. C Scatter plot depicting the transcript levels of miR-137 in control and GBM samples in TCGA dataset. D Dot plot represents the correlation between expression of PTGFRN and miR-137 in TCGA GBM samples. E The bar graph shows the transcript levels of miR-137 in IHA and GBM cell lines. F The correlation graph shows the correlation between protein levels of PTGFRN (normalized values from Fig. 1I) and the miR-137 levels (log₂ ratio values from Fig. 3E) in GBM cell lines. G Schematic shows miR-137 targeting sites on the 3’UTR of PTGFRN and base pairing between miR-137 and targeted sequence in the 3’UTR of PTGFRN. (H) Immunoblot shows PTGFRN protein levels in vector and miR-137 overexpression in U373 and β-Actin was used as a loading control (required portion of the blot is shown after cropping from the whole blot for both the proteins). I The bar graph is depicting the normalized luciferase activity of pmiR-GLO-3’UTR of PTGFRN in pcDNA3.2/V5-Vector and pcDNA3.2/V5-miR-137 overexpression conditions. The significance was performed using the Wilcoxon-Mann-Whitney test or Student's t-test and the symbols are indicated as follows: (ns) not significant; (*) p ≤ 0.05; (**) p ≤ 0.01 and (***) p ≤ 0.001