Fig. 7
From: LukS-PV inhibits hepatocellular carcinoma cells migration by downregulating HDAC6 expression
LukS-PV inhibited HCC cells migration by increasing α-tubulin acetylation through HDAC6 downregulation. A–B Huh-7 and Hep3B cells containing EV (empty vector) or overexpressing HDAC6 (HDAC6 OE) were transfected with either the α-tubulin K40Q or K40R mutant plasmids and treated with LukS-PV. The mobility of cells was examined by scratch assays. The percentage of relative migration was calculated. C-D The protein expression levels of Ac-α-tubulin, α-tubulin, MMP2 and MMP9 were detected by western blot. Relative band intensities (compared to GAPDH) were measured using Image J. * P < 0.05, ** P < 0.01, or *** P < 0.001