Fig. 4

NOSTRIN curtails invasiveness of colon cancer cells. A Photomicrographs of colonies formed in soft agar by control and NOSTRIN overexpressing HCT 116 cells. Scale bar: 100 μm, Magnification: 50X. B Fluorometric quantification of relative colony formation ability in soft agar of control and NOSTRIN over-expressing HCT116 cells. Data is representative of three independent biological replicates. Error bars represent standard error of mean. C Photomicrograph of invaded control and NOSTRIN over-expressing HCT116 cells from cell invasion assay. Scale bar: 100 μm, Magnification: 100X. D Invaded cell count per microscopic field. E Colorimetric quantification of the invaded cells at the lower surface of the membrane (n = 3). F Representative photo micrographic images depicting wound closure ability of control and NOSTRIN over-expressing HCT116 cells. Magnification: 50X. G Quantification of percentage wound closure from a minimum of 5 measurements in each experiment using three different biological replicates. Error bars represent standard error of mean from three biological replicates. **p < 0.01, ***p < 0.001