Fig. 1

NOSTRIN expression is inversely related to aggressiveness of colon cancer cells. A Quantitative real time PCR analysis of Nostrin transcript in colon cancer cell lines HT29, HCT116. B Western blot analysis of NOSTRIN protein levels in colon cancer cell lines HT29, HCT116. The bar graph adjacent to the blot represents quantification of NOSTRIN protein levels normalized to endogenous control GAPDH using NIH ImageJ software. Full length blots are shown in Fig. S3. C Photomicrograph of invaded HT29 and HCT116 cells from cell invasion assay. Scale bar: 100 μm, Magnification: 100X. D Invaded cell count per microscopic field. E. Colorimetric quantification of the invaded cells at the lower surface of the membrane (n = 3). F Representative photo-micrographic images depicting wound closure ability of HT29 and HCT116 cell lines. Magnification used, 50X. G Quantification of percentage wound closure from a minimum of 5 measurements in each experiment using three different biological replicates. H Photomicrographs of colonies formed in soft agar by HT29 and HCT 116 cells. Scale bar: 100 μm, Magnification: 50X. I Fluorometric quantification of relative colony formation ability in soft agar of HT29 and HCT116 cells. Data is representative of three independent biological replicates. Error bars represent standard error of mean from three biological replicates. *p < 0.05, ** p < 0.01, ***p < 0.001