Fig. 4
From: The identification and validation of EphA7 hypermethylation, a novel biomarker, in cervical cancer
Increased DNA methylation of EphA7 by the dCas9-DNMT3a system. a qRT–PCR demonstrated that EphA7 mRNA levels were downregulated compared with DNMT3am (DNMT3am was DNMT3a mutations) after cotransfection of 4 repression sgRNAs with the dCas9-DNMT3a in HEK293T cells (n = 3). b Pyrosequencing showed that the methylation level in the dCas9-DNMT3a group were higher than the control (n = 3). c The average CpG sites of the promoter increased nearly 23.67% (n = 3) in the dCas9-DNMT3a group