Fig. 3

Paclitaxel treatment induces ovarian PGCC formation. a, b and c SKOV3 cells were treated with 0, 1, 10 and 100 nM paclitaxel (Tx) for 48 h. a The cells were labeled with DAPI (nucleus, blue) and Phalloidin (actin, red) and observed by fluorescence microscopy at the original magnification of 40×. The white arrow indicates the multinucleated cell. b Protein levels for GRP78 and GAPDH were assessed by Western blotting. GRP78 and GAPDH (used as loading control) were quantified using the ImageJ software, and data are expressed as the GRP78/GAPDH fold change relative to the control. c Fusion index and cell viability (MTT assay) were evaluated. A correlation between fusion index and paclitaxel treatment or cell viability was then calculated. R: Pearson correlation. d Co-culture of SKOV3-Green and SKOV3-Red cells was treated with 10 nM Paclitaxel for 48 h. c The presence of polynucleated cells with both red and green nuclei was detected by fluorescence microscopy (delimited with a white dashed line). The picture represents the overlay of red signal (mCherry), green signal (GFP) and phase contrast. The white boxed area is shown at a higher magnification to the right and (X3) and brightness. Scale bar, 200 μm; original magnification, × 20