Fig. 3

Integrin α6β4 promoted Notch signals activation. A, relative expression of AKT1, STAT3, Wnt3A, Notch1, c-Myc and SOX2 in Biu-87 cells cultured on dish and 2D laminin/collagen gels. B, western blotting of cleaved intracellular domain of Notch1 in Biu-87 and MB49 cells cultured on dish or 2D laminin/collagen gels. C, western blotting of cleaved intracellular domain of Notch1 in Biu-87 cells cultured on 2D laminin/collagen gels (treated with scrambled siRNA, ITGA6 siRNA and ITGB4 siRNA). D, the full length human Notch1 promoter was cloned into the luciferase reporter vector. Notch1 transcription activity was examined after dish or 2D collagen/laminin culture (3 days) in Biu-87 and MB49 cells. E, cell proliferation of dish and 2D laminin/collagen cultured Biu-87/MB49 cells treated with PBS or SAHM1 (10 nM). F, relative migrating cells of dish and 2D laminin/collagen cultured Biu-87/MB49 cells treated with PBS or SAHM1 (10 nM). G, survival analysis of bladder cancer patients with high/low Notch1 expression using TCGA database (n = 405)