Fig. 5

TESC interacted with c-Fos through ERK1/2 in TPC-1 and BHT101 cells. A The relative protein level of ERK1/2 and phosphorylated ERK1/2 was examined by western blot assay in TPC-1 and BHT101 cells treated with TESC-RNAi or FOS-RNAi. B The relative protein level of ERK1/2 and phosphorylated ERK1/2 was shown as a bar graph in TPC-1 and BHT101 cells treated with TESC-RNAi or FOS-RNAi. C Overexpressed plasmid of TESC was transfected into TPC-1 and BHT101 cells, and then cells were treated with ERK inhibitor AG-126. The proteins expression of c-Fos, ERK1/2 and phosphorylated ERK1/2 was examined by western blot assay. D The relative protein level of c-Fos, ERK1/2 and phosphorylated ERK1/2 was shown as a bar graph. Data in western blot assay were expressed after being normalized to β-actin. The means ± SD of three independent samples were shown. *p < 0.05