Fig. 3

A directly positive correlation between TESC and c-Fos in TPC-1 and BHT101 cells. The mRNA and protein level of c-Fos were determined after the expression of TESC was downregulated and upregulated in TPC-1 and BHT101 cells respectively. A The relative mRNA level of c-Fos was detected using qRT-PCR after the inhibition of TESC in TPC-1 and BHT101 cells. B The relative protein level of c-Fos was shown as a bar graph after the inhibition of TESC in TPC-1 and BHT101 cells. C The proteins expression of c-Fos was examined by western blot assay after the inhibition of TESC in TPC-1 and BHT101 cells. D The relative mRNA level of c-Fos was detected using qRT-PCR after the overexpression of TESC in TPC-1 and BHT101 cells. E The relative protein level of c-Fos was shown as a bar graph after the overexpression of TESC in TPC-1 and BHT101 cells. F The proteins expression of c-Fos was examined by western blot assay after the overexpression of TESC in TPC-1 and BHT101 cells. Data in qRT-PCR assay were expressed after being normalized to GAPDH. Data in western blot assay were expressed after being normalized to β-actin. The means ± SD of three independent samples were shown. *p < 0.05