Fig. 1
TESC effectively promoted the proliferation, migration, invasion, and inhibited the apoptosis of PTMC. A The expression of TESC in PTMC tissues and PTMC-adjacent tissues was detected by IHC, and the expression change of TESC was statistically analyzed. B The mRNA expression of TESC in the cells was detected using qRT-PCR. C Three target RNAi sequences of TESC were cloned into GV493 vector, and then were transfected into TPC-1 and BHT101 cells using Polybrene, respectively. The interference efficiency was determined by qRT-PCR. D and E The effect of TESC on the proliferation of TPC-1 and BHT101 cells was detected by CCK8 and colony formation assay. F The apoptosis rate of TPC-1 and BHT101 cells was determined by flow cytometric assay. G The effect of TESC on the migration of TPC-1 and BHT101 cells was examined by scratch test. H The effect of TESC on the invasion abilities of TPC-1 and BHT101 cells was examined by transwell assay. I and J TESC-RNAi transfected (a total of 2 × 106) or control cells were subcutaneously injected into the flanks of nude mice. Tumor volume and weight were measured. K The level of Ki-67 was detected by IHC after the inhibition of TESC. The means ± SD of three independent samples were shown. *p < 0.05