Fig. 9

PFKFB4 overexpression promotes the growth and metastasis of OS cells. A transfection efficacy of pCAG-PFKFB4 in OS cells examined by RT-qPCR; B proliferative activity of MG63 and HOS cells detected by the CCK-8 assay; C colony formation ability of MG63 and HOS cells examined by the colony formation assay; D migration of MG63 and HOS cells examined by the scratch test; E invasion of MG63 and HOS cells examined by the Transwell assay; F growth rate of xenograft tumors formed by HOS cells in nude mice; G weight of the xenograft tumors on day 20; H tumor infiltration in mouse lung tissues 30 days after tail vein injection of HOS cells examined by HE staining. For cellular experiments, three repetitions were performed; for animal experiments, n = 5 in each group. Data were analyzed by the unpaired t test (G-H) and two-way ANOVA (A-F) followed by Tukey’s test. *p < 0.05