Fig. 7

KDM3A-SP1 mediates PFKFB4 expression. A potential regulation of SP1 on several common key enzymes related to glycolysis predicted in the hTFtarget system; B correlations of SP1 with PFKGB4 and HK2 in sarcoma predicted in the GEPIA system; C IHC score of PFKFB4 in the collected tumor and peritumor bone tissue samples; D correlation between SP1 and PFKFB4 IHC scores in OS tissues; E-F, PFKFB4 mRNA (E) and protein (F) levels in OS cells transfected with sh-KDM3A/pCAG-SP1 determined by RT-qPCR and western blot analysis, respectively. Three independent experiments were performed. Differences were analyzed by the paired t test (C) and two-way ANOVA (E-F) followed by Tukey’s test. Correlation between variables was analyzed by the Pearson’s correlation analysis (D). *p < 0.05