Fig. 4

KDM3A mediates H3K9me2 modification on SP1 promoter to regulate its expression. A genes positively correlated with KDM3A in sarcoma tissues in UALCAN system; B, C KEGG pathway enrichment analysis on the candidate target genes of KDM3A; D expression profiles of SIN3A and SP1 in sarcoma in the GEPIA database; E expression patterns of SIN3A and SP1 in Ewing Sarcoma and OS in the GEO GSE73166 dataset; F, G mRNA (F) and protein (G) expression of SIN3A and SP1 in OS cells transfected with sh-KDM3A examined by RT-qPCR and western blot analysis, respectively; H IHC score of SP1 in the collected tumor and peritumor bone tissue samples; I correlation between KDM3A and SP1 IHC scores in OS tissues; J enrichment of KDM3A on SP1 promoter; K KDM3A and H3K9me2 modification levels on SP1 promoter in OS cells following KDM3A inhibition examined by the ChIP-qPCR assay. Three independent experiments were performed. Differences were analyzed by the unpaired t test (E), paired t test (H), and two-way ANOVA (F, G and K) followed by Tukey’s test. Correlation between variables was analyzed by the Pearson’s correlation analysis (I). *p < 0.05