Fig. 10

PFKFB4 enhances glycolytic flux in OS cells. F6P production (A), G3P production (B), glucose uptake (C), lactate production (D), ECAR (E) and OCR (F) in OS cells after PFKFB4 overexpression; G protein levels of PFKFB4 in cells determined by western blot analysis; H proliferative activity of MG63 and HOS cells detected by the CCK-8 assay; I colony formation ability of MG63 and HOS cells determined by the colony formation assay; J migration of MG63 and HOS cells examined by the scratch test; K invasion of MG63 and HOS cells examined by the Transwell assay. Three independent experiments were performed. Differences were analyzed by the two-way ANOVA (A-K) followed by Tukey’s test. *p < 0.05