Fig. 2
From: Stimulating TAM-mediated anti-tumor immunity with mannose-decorated nanoparticles in ovarian cancer
MnNP Biodistribution in ID8 Tumor Model. C57Bl/6 mice bearing ID8 ovarian tumors were treated IP with Cy5-MnNPs. After 24 hours, the ascites, tumors, and spleens were collected for flow cytometry analysis. A The general cell population gated on FSC vs. SSC revealed a high percentage of MnNP+ cells in the ascites and almost 20% of cells in the tumor, but negligible delivery to any cells in the spleen. B Gating on CD45+/F4/80+ macrophages revealed that about 60% of macrophages in the ascites and ~ 75% of TAMs were MnNP+. Additional gating for (C) Gr-1- mature macrophages and (D) Gr-1+ myeloid cells also revealed high percentages of MnNP uptake in the ascites and tumor (n = 3, *p < 0.05 **p < 0.001)