Fig. 3
From: miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer
MiR-107 modulates the expression of NEDD9. a Common miRNAs predicted by TargetScan, DIANA and miRanda to regulate NEDD9. b TargetScan software was used to predict the binding sites of miR-103a-3p, miR-107 and NEDD9. c qPCR analysis of the expression of miR-107 and miR-103a-3p in MCF-7 and MDA-MB-231 cells. However, only miR-107 was differentially expressed. d Construction of fluorescein-expressing wild-type NEDD9 plasmids and fluorescein-expressing plasmids encoding NEDD9 with mutations at the miR-107-binding site. e and f Transfection of the fluorescein-expressing wild-type NEDD9 plasmids and fluorescein-containing plasmids encoding NEDD9 with mutations at the miR-107-binding site, miR-107 mimic and NC mimic into 293 T cells. Binding was detected based on luciferase activity (n = 3). ***P < 0.001, *P < 0.05