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Fig. 1 | BMC Cancer

Fig. 1

From: DAB2IP down-regulates HSP90AA1 to inhibit the malignant biological behaviors of colorectal cancer

Fig. 1

DAB2IP negatively regulates the expression of HSP90AA1 in CRC. a The mRNA expression level of DAB2IP in human normal tissues and colorectal cancer in the TCGA database. Wilcox test was used, *P < 0.05, ** P < 0.01, ***P < 0.001 and P < 0.05 was considered statistically significant. b The mRNA expression level of HSP90AA1 in human normal tissues and colorectal cancer in the TCGA database. Wilcox test was used, *P < 0.05, ** P < 0.01, ***P < 0.001 and P < 0.05 was considered statistically significant. c The mRNA expression level of DAB2IP in human normal colon tissue and colorectal cancer in GSE8671. Wilcox test was used, *P < 0.05, ** P < 0.01, ***P < 0.001 and P < 0.05 was considered statistically significant. d The mRNA expression level of HSP90AA1 in human normal colon tissue and colorectal cancer in GSE8671. Wilcox test was used, *P < 0.05, ** P < 0.01, ***P < 0.001 and P < 0.05 was considered statistically significant. e The mRNA expression levels of DAB2IP in normal colon epithelial cells NCM460 and CRC cell lines SW480, HT29, HCT116, and DLD1 were detected by quantitative PCR. f The mRNA expression levels of HSP90AA1 in normal colon epithelial cells NCM460 and CRC cell lines SW480, HT29, HCT116, and DLD1 were detected by quantitative PCR. The above RT-PCR data were expressed as mean ± SEM, n = 3; * p < 0.05, ** p < 0.01;*** p < 0.001. g Spearman correlation analysis of the expression of DAB2IP and HSP90AA1 in the CRC dataset "Tumor Colon—Siebersmith 355-MAS5.0-U133P2" in the R2 database. h Spearman correlation analysis of DAB2IP and HSP90AA1 expression in 19 human CRC samples. i Protein expression levels of DAB2IP and HSP90AA1 proteins in 4 pairs of CRC tissues and non-tumor colon tissues of patients. j Statistical analysis of protein gray value, which was quantified by Image J software. Two-sided student’s t-tests was used, * p < 0.05, ** p < 0.01;*** p < 0.001. k The effect of DAB2IP overexpression on the expression of HSP90AA1 was detected by PCR and Western blotting. m n Changes in HSP90AA1 expression after knocking down DAB2IP were detected by PCR and Western blotting

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