Fig. 2

Serotonin drove glioma cells proliferation and invasion. A and B 10⁵ LN229/T98G (VEC) or TPH-1 overexpressing LN229/T98G cells (OE) were cultured in DMEM culture medium for 48 h. Trp consumption (A) and serotonin production (B) was determined by Elisa assay. C cell proliferation of LN229/T98G cells treated with PBS or serotonin (10 nM). D relative migrating cells of LN229/T98G cells treated with PBS or serotonin (10 nM). E LN229 or T98G cells were pre-treated with PBS or serotonin (10 nM) for 48 h. Then cell apoptosis of LN229/T98G treated with PBS or TMZ (48 h) were determined. F immunofluorescence staining of TPH-1 in TPH-1^high and TPH-1^low glioma tissues from patients. The scale bar was 25 μm. G serotonin secretion in TPH-1^high and TPH-1^low glioma tissues from patients, detected by Elisa analysis. H the correlation analysis between TPH-1 and serotonin in 20 glioma tissues (R² = 0.466)