Fig. 6

SNCA affects proliferation of LUAD cells in vitro via inhibiting PI3K/AKT/mTOR signaling pathway. A Proliferation of A549 cells overexpressing SNCA was assessed by CCK8; *** P < 0.001. B Effect of SNCA on A549 cell growth were evaluated by EdU assay (n = 5). Results of quantitative analysis were measured by Image J; **** P < 0.0001. C Effects of SNCA on A549 cell growth were further evaluated using a colony formation assay. Quantitative analysis of colony numbers is measured by Image J; ** P < 0.01. D Levels of PI3K, AKT, p-AKT, mTOR and p-mTOR proteins were assessed in stably-transfected A549 cells by Western blotting; ** P < 0.01, *** P < 0.001