Fig. 5

LMOD1 promoted peritoneal metastasis of gastric cancer cells by regulating the epithelial-mesenchymal transition. A, B GSEA analysis of LMOD1 enriched pathways in TCGA and GSE62254 databases. C, D Western blotting was used to verify the regulation of epithelial-mesenchymal transition (EMT)-related proteins in Control or shLMOD1 SGC-7901 and MGC-803 cell lines. E Western blotting was used to verify the regulation of EMT-related proteins in Control/\ or overexpressed-LMOD1 HGC-27 cell lines. F-H The adhesion ability of cells was verified using an adhesion test. I-K An invasion assay was used to verify the ability of peritoneal infiltration. L, M The effect of LMOD1 silencing on invasion and migration of gastric cancer cells in SGC-7901 cells was verified by intraperitoneal metastasis model in nude mice. We cut the membrane to facilitate the better hybridization of antibodies and avoid the interaction of antibodies. *, P < 0.05, **, P < 0.01, ***, P < 0.001, ****, P < 0.0001