Fig. 3

The combination of Sorafenib and CT-707 induced enhanced apoptosis of hepatocellular carcinoma cells. A SMMC-7721 cell was treated with sorafenib (15 μM), CT-707(4 μM) or both, and the cell apoptosis was detected by AV/PI staining (the units of the y-axis and x-axis are fluorescence intensity. y-axis: PI staining; x-axis: Annexin V). B SMMC-7721 was treated with sorafenib (20 μM), CT-707(3 μM) or both, and the protein expression levels of cleaved PARP (c-PARP) and β-actin in the cell lines were determined using western blot analysis. Full-length blots/gels are presented in Supplementary materials Figure 1A