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Fig. 3 | BMC Cancer

Fig. 3

From: AXL regulates neuregulin1 expression leading to cetuximab resistance in head and neck cancer

Fig. 3

HER3 overexpression alone is insufficient for cetuximab resistance. A: HN30 and PCI37A cells stably overexpressing HER3 or the pcDNA6.0 vector were treated with 100 nM of cetuximab (Ctx) for 72 h before performing crystal violet assay for HN30 cells and CCK8 assays for PCI37A cells. Whole cell lysate was harvested at 24 h after treatment and subjected to immunoblot analysis following fractionation via SDS-PAGE. GAPDH or α-Tubulin was used as a loading control. Mean values, SEs, and statistical analyses are representative of two or three independent experiments. N = 3–6. NS: not significant

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