Fig. 1

AXL leads to cetuximab resistance and increased HER3 activity. A: HN30 cells were treated with 100 nM of IgG, 100 nM of cetuximab (Ctx), or combination of Ctx and Gas6 (200 ng/uL) for 72 h, and cell viability was determined by crystal violet assay. Mean values, SEs, and statistical analyses are representative of two independent experiments. N = 3, **P < 0.01. Whole cell lysates were harvested and fractionated via SDS-PAGE, followed by immunoblotting for the indicated proteins. GAPDH was used as a loading control. B: Cell viability in AXL overexpressed cells was measured via crystal violet assay after 72 h of treatment with cetuximab. Mean values, SEs, and statistical analyses are representative of three independent experiments. N = 3, **P < 0.01. Whole cell lysates were harvested and fractionated via SDS-PAGE, followed by immunoblotting for the indicated proteins. α-Tubulin and GAPDH were used as loading controls