Fig. 5
From: OLA1 promotes colorectal cancer tumorigenesis by activation of HIF1α/CA9 axis
The differential expression of HIF1α and CA9 in Ctrl and OLA1-KO CRC cell lines. A CA9 mRNA expression was heavily downregulated in OLA1-KO CRC cell lines by real-time qRT-PCR (P < 0.001). B CA9 protein was downregulated and GSK3β was upregulated in OLA1-KO CRC cell lines by western blot (the blots in this figure were cut prior to hybridization with antibody GSK3β, P- GSK3β, GAPDH and OLA1). C In OLA1-KO mouse E18.5 embryo tissues, GSK3β was upregulated compared with the control tissues. The number 1 to 12 represent 12 different tissues from 12 embryos. Number 1 to 7, 8 to 9, and 10 to 12 from 3 mothers separately