Fig. 2
From: OLA1 promotes colorectal cancer tumorigenesis by activation of HIF1α/CA9 axis
OLA1 knockout inhibits proliferation in vitro and in vivo. A Immunoblotting analysis of OLA1 protein level in control and OLA1-KO cell lines (Hct116 and Lovo cells, the blots in this figure were cut prior to hybridization with antibody GAPDH and OLA1); B, C CCK-8 assay for investigating proliferation potential of Ctrl and OLA1-KO cell lines, (B) Hct116 and (C) Lovo. D Colony formation assay for investigating colony formation ability of Ctrl and OLA1-KO cell lines; E Xenograft model of BALB/c-nu/nu female mice with subcutaneous injection Hct116 and Hct116-OLA1-KO cells(left-up), Lovo and Lovo-OLA1-KO cells(left-down), tumor volume when mice were executed in xenograft model (right). Data are presented as mean ± SD; statistical significance was assessed by unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Ctrl, scramble; OLA1-KO, OLA1 knockout