Fig. 6

Autophagy-modulating therapeutics alter the expression of GRP78 and LC3B-II. CAOV3 cells were treated for 72 h with vehicle control or HCQ (A), NFV(B), or Rapa (C) and concurrently with and without CPt or DTx as indicated and western blotting for GRP78, actin, and LC3B was conducted as described in Methods. LC3B runs as a doublet; the upper band is free cytosolic LC3B-I and the lower band is lipidated LC3B-II present in autophagosomes. Blots are representative of two independent experiments. Full-length blots are presented in Supplementary Fig. 1