Fig. 5

Rapamycin (Rapa) does not increase PGCC formation in CAOV3 or OVCAR3 cells. A CAOV3 cells were treated with 10 nM Rapa or vehicle control for 72 h and allowed to recover in fresh drug-free media for 72 h prior to PGCC quantitation by Hoescht 33342 staining. B CAOV3 cells were co-treated with CPt and Rapa or vehicle control for 72 h and allowed to recover in fresh drug-free media for 72 h prior to PGCC quantitation. C CAOV3 cells were co-treated with DTx and Rapa or vehicle control for 72 h and allowed to recover in fresh drug-free media for 72 h prior to PGCC quantitation. D-F Identical experiments as in (A-C) were performed using OVCAR3 cells. P-values indicated Fisher’s exact test result, and 100 cells quantified from two independent experiments are shown