Table 2 Primers used to study promoter hypermethylation of different genes
Gene | Name | Primer sequence |
|---|---|---|
DAPK1 | Unmethylated forward | 5′-GGAGGATAGTTGGATTGAGTTAATGTT-3′ |
Unmethylated Reverse | 5′-CAAATCCCTCCCAAACACCAA-3′ | |
Methylated forward | 5′-GGATAGTCGGATCGAGTTAACGTC-3′ | |
Methylated Reverse | 5′-CCCTCCCAAACGCCGA-3′ | |
RIZ-1 | Unmethylated forward | 5′-TGGTGGTTATTGGGTGATGGT-3′ |
Unmethylated Reverse | 5′-ACTATTTCACCAACCCCAAGA-3′ | |
Methylated forward | 5′-GTGGTGGTTATTGGGCGACGGC-3′ | |
Methylated Reverse | 5′-GCTATTTCGCCGACCCCGACG-3′ | |
P16INK4A | Unmethylated forward | 5′-TTATTAGAGGGTGGGGTGGATTGT-3′ |
Unmethylated Reverse | 5′-CAACCCCAAACCACAACCATAA -3′ | |
Methylated forward | 5′-TTATTAGAGGGTGGGGCGGATCGC-3′ | |
Methylated Reverse | 5′-GACCCCGAACCGCGACCGTAA -3′ | |
RASSF1A | Unmethylated forward | 5′-GGGTTTTGCGAGAGCGCGT-3′ |
Unmethylated Reverse | 5′-GCTAACAAACGCGAACCG-3′ | |
Methylated forward | 5′- GGTTTTGTGAGTGTGTTTAGT-3′ | |
Methylated Reverse | 5′-CACTAACACACAAACCAAACA-3′ | |
SOCS1 | Unmethylated forward | 5′-TCGTTCGTACGTCGATTATC-3′ |
Unmethylated Reverse | 5′-AAAAAAATACCCACGAACTCG-3′ | |
Methylated forward | 5′-TATTTTGTTTGTATGTTGATTATTG-3′ | |
Methylated Reverse | 5′-AAACTCAACACACAACCACTC-3′ | |
p14ARFARF | Unmethylated forward | 5'-TTTTGGTGTTAAAGGGTGGTGTAGT-3 ‘ |
Unmethylated Reverse | 5'- CACAAAAACCCTCACTCACAACAA-3' | |
Methylated forward | 5'-GTGTTAAAGGGCGGCGTAGC-3’ | |
Methylated Reverse | 5'- AAAACCCTCACTCGCGACGA-3' |