Fig. 5

Demethylation promotes the expression of miR-138 and inhibits cervical tumor growth in vivo. a Images of tumors grown in nude mice transplanted with cervical cancer cells treated with decitabine (DAC), the miR-138 agomir, or negative-control miRNA (NC, N = 5 in each group). b Tumor growth curves of mice in the three groups. Tumor volumes were measured every 2 weeks starting 7 days after injection. c Tumor weights in all mice. Data are presented as the mean ± standard deviation. d Quantitative RT-PCR analysis was applied to determine the relative levels of miR-138 in all tumors described in (a). e Representative images of immunohistochemical staining of enhancer of zeste homolog 2 (EZH2), E-cadherin, N-cadherin, and vimentin in tumor samples described in (a). f Western blot analysis of EZH2, E-cadherin, N-cadherin, and vimentin expression in tumor samples described in (a). Three samples in each group were randomly selected for analysis. GAPDH served as a loading control