Fig. 7
From: HOXA-AS2 enhances GBM cell malignancy by suppressing miR-2116-3p thereby upregulating SERPINA3
SERPINA3 was a target of miR-2116-3p. A The potential binding sites between miR-2116-3p and SERPINA3. B Dual-luciferase reporter assay was performed to verify the relationship between miR-2116-3p and SERPINA3. **P < 0.001 compared with miR-2116-3p mimic NC. C SERPINA3 could be obviously pulled down by miR-2116-3p mimic showed by RNA pull-down analysis. **P < 0.001 compared with bio-mimic-NC. D Upregulation of SERPINA3 in GBM tissues. **P < 0.001 compared with normal tissues. (E) Negative correlation between miR-2116-3p and SERPINA3 expression. F Upregulation of SERPINA3 in U251 and A172 cells. **P < 0.001 compared with NHA. G The transfection efficiency of si-SERPINA3 in U251 and A172 cells by western blotting. *P < 0.05, **P < 0.001 compared with blank group. #P < 0.05, ##P < 0.001 compared with Si-SERPINA3 + inhibitor group. Data were present as mean ± SD