Fig. 2

Analysis of hBMSC and PC-3 by histology and flow cytometry. (a, b) Quantification of hBMSC-Luc/GFP in femur histology slices. (a) Histology 40x magnification image of marrow with anti-GFP (green) and DAPI (blue) to detect hBMSC-Luc/GFP. Scale bar = 20 μm. (b) Comparison of relative hBMSC-Luc/GFP numbers in histology slices at 6 weeks (PC-3-DsRed n = 4, hBMSC-Luc/GFP + PC-3-DsRed n = 6). Flow cytometry quantification of PC-3-DsRed numbers in (c) mouse contralateral and (d) injected femurs. Gating identified live singlet cells, which were negative for mouse CD45, but positive for a DsRed signal (Supplementary Fig. 9). (e) Quantification of total PC-3-DsRed numbers taking the average of both femurs, in animals that either did or did not receive hBMSC-Luc/GFP. Statistics determined by the Mann-Whitney t-test detected a significant difference (p = 0.0445) in the number of PC-3-DsRed in animals that had been transplanted with hBMSC-LUC/GFP. (f) Comparison of the distribution of PC-3-DsRed between femurs in individual mice femurs. Individual flow images are found in Supplementary Fig. 10. Mann Whitney t-test did not identify difference between injected vs non-injected femur (PC-3-DsRed, p = 0.6589; hBMSC-Luc/GFP + PC-3-DsRed, p = 0.5223). Two flow experiments pooled, (no cells n = 2, PC-3-DsRed only n = 8, hBMSC-Luc/GFP only n = 7, hBMSC-Luc/GFP + PC-3-DsRed n = 11).