Fig. 3

Primary prostate adenocarcinoma cell lines disseminate in-vivo. A xenograft formation in zebrafish yolk-sac invasion assay including an example of a non xenografting cell line NIH 3T3. B metastatic dissemination in the tail 3 days post injection, indicated by arrows. C Quantification of the percentage of embryos exhibiting metastasis in the tail region. Data are representative of three independent experiment, with at least 15 embryos screened for metastasis. Data are presented as mean ± S.E.M. Significance was calculated with One-way Anova followed by Tukey’s multiple comparisons test. Scale bar = 200 µm