Skip to main content
Fig. 3 | BMC Cancer

Fig. 3

From: Circ_0058063 contributes to cisplatin-resistance of bladder cancer cells by upregulating B2M through acting as RNA sponges for miR-335-5p

Fig. 3

Circ_0058063 positively regulated B2M expression via sponging miR-335-5p. A The binding sites of circ_0085063/B2M (wild and mutant) and miR-335-5p were predicted by Starbase online database. B circ_0058063 and miR-335-5p co-immunoprecipitated with Ago2 revealed by RIP assay. C-F Dual-luciferase reporter assay examined the luciferase activity of wild/mutant circ_0058063 and B2M by miR-335-5p mimic or NC mimic. G, H The mRNA levels of miR-335-5p and B2M in SV-HVC-1, T24, T24/CDDP, 5637 and 5637/CDDP cell lines. I The mRNA levels of miR-335-5p and B2M in CDDP-resistant (n = 19) and CDDP-sensitive bladder cancer tissues (n = 16). J, K Western blot detected that the B2M protein expression was significantly increased in T-24/CDDP and 5637/CDDP cells compared with that in T24 and 5637 cells. L The B2M mRNA expression levels after knockdown or overexpression of circ_0058063 by RT-qPCR. M, N The B2M protein expression levels after knockdown or overexpression of circ-_0058063 by western blot. *P < 0.05

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us