Fig. 3

Transcriptomics and proteomics analysis of chemotherapy and radiotherapy in sensitive and resistant CRC cell lines. A Heat maps of the proteomics data from sensitive (S) and resistant (R) DLD-1 (D), HCT-116 (116) and HT-29 (29) CRC cell lines treated with uracil (U), 5-FU alone (5) or with irradiation (5I) (Perseus 1.6.12.0) [74]. B Representation of the number of significant up- (light) and down-regulated (dark) proteins for each CRC cell line after a treatment with 5-FU alone (+5FU, purple) or with irradiation (+5FU + IRR, yellow) compared to the control condition (uracil) and after the induction of a chemoresistance (S vs R, green). A minimum absolute z-score of 2 (calculated from the ratio treated/control) was used to determine significance. Venn diagrams (merge) highlight the common modulated proteins between the three conditions for each CRC cell line (Venny 2.1.0) [70]. C PCA analysis of the RNA seq data from sensitive (S) and resistant (R) DLD-1 (D), HCT-116 (116) and HT-29 (29) CRC cell lines treated with uracil (U), 5-FU alone (5) or with irradiation (5I). The biological triplicates for each sample are grouped by colors. The two main components PC1 and PC2 were used for two-dimensional visualization of the analysis. D Representation of the number of significant up- (light) and down-regulated (dark) transcripts for each CRC cell line after a treatment with 5-FU alone (+5FU, in purple) or with irradiation (+5FU + IRR, in yellow) compared to the control condition (uracil) and after the induction of a chemoresistance (S vs R, in green). A minimum absolute fold change of 2 combined with an adjusted p-value threshold ≤0.01 was used to determine significance. Venn diagrams (merge) highlight the common modulated transcripts between the three conditions for each CRC cell line (Venny 2.1.0) [70]